Composite

Part:BBa_K5250020

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter + T7-like RNAP

Transcription unit that expresses T7-like RNAP (BBa_K5250002) constitutively. It is part of the positive feedback loop we are implementing in our construct. Its aim is to enhance the production of our gene of interest. It is specifically tailored for expression in Pseudomonas species, since it originates from the Pseudomonas phage Pf-10.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal SapI site found at 2519
    Illegal SapI.rc site found at 1714


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Categories
Parameters
None